Large-field lattice structured illumination microscopy.
Opt Express
; 30(15): 27951-27966, 2022 Jul 18.
Article
in En
| MEDLINE
| ID: mdl-36236953
ABSTRACT
In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMD-based SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lighting
Language:
En
Journal:
Opt Express
Journal subject:
OFTALMOLOGIA
Year:
2022
Document type:
Article