A Toolkit for Precise, Multigene Control in Saccharomyces cerevisiae.
ACS Synth Biol
; 11(12): 3912-3920, 2022 12 16.
Article
in En
| MEDLINE
| ID: mdl-36367334
ABSTRACT
Systems that allow researchers to precisely control the expression of genes are fundamental to biological research, biotechnology, and synthetic biology. However, few inducible gene expression systems exist that can enable simultaneous multigene control under common nutritionally favorable conditions in the important model organism and chassis Saccharomyces cerevisiae. Here we repurposed ligand binding domains from mammalian type I nuclear receptors to establish a family of up to five orthogonal synthetic gene expression systems in yeast. Our systems enable tight, independent, multigene control through addition of inert hormones and are capable of driving robust and rapid gene expression outputs, in some cases achieving up to 600-fold induction. As a proof of principle, we placed expression of four enzymes from the violacein biosynthetic pathway under independent expression control to selectively route pathway flux by addition of specific inducer combinations. Our results establish a modular, versatile, and potentially expandable toolkit for multidimensional control of gene expression in yeast that can be used to construct and control naturally occurring and synthetic gene networks.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Synthetic Biology
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
ACS Synth Biol
Year:
2022
Document type:
Article
Affiliation country:
Estados Unidos