Your browser doesn't support javascript.
loading
tRNA abundance, modification and fragmentation in nasopharyngeal swabs as biomarkers for COVID-19 severity.
Katanski, Christopher D; Alshammary, Hala; Watkins, Christopher P; Huang, Sihao; Gonzales-Reiche, Ana; Sordillo, Emilia Mia; van Bakel, Harm; Lolans, Karen; Simon, Viviana; Pan, Tao.
Affiliation
  • Katanski CD; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, United States.
  • Alshammary H; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Watkins CP; Center for Vaccine Research and Pandemic Preparedness (C-VARPP), Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Huang S; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, United States.
  • Gonzales-Reiche A; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, United States.
  • Sordillo EM; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • van Bakel H; The Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Lolans K; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Simon V; Icahn Genomics Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
Front Cell Dev Biol ; 10: 999351, 2022.
Article in En | MEDLINE | ID: mdl-36393870
Emerging and re-emerging respiratory viruses can spread rapidly and cause pandemics as demonstrated by the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The early human immune responses to respiratory viruses are in the nasal cavity and nasopharyngeal regions. Defining biomarkers of disease trajectory at the time of a positive diagnostic test would be an important tool to facilitate decisions such as initiation of antiviral treatment. We hypothesize that nasopharyngeal tRNA profiles could be used to predict Coronavirus Disease 19 (COVID-19) severity. We carried out multiplex small RNA sequencing (MSR-seq) on residual nasopharyngeal swabs to measure simultaneously full-length tRNA abundance, tRNA modifications, and tRNA fragmentation for the human tRNA response to SARS-CoV-2 infection. We identified distinct tRNA signatures associated with mild symptoms versus severe COVID-19 manifestations requiring hospitalization. These results highlight the utility of host tRNA properties as biomarkers for the clinical outcome of SARS-CoV-2.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Front Cell Dev Biol Year: 2022 Document type: Article Affiliation country: Estados Unidos Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Front Cell Dev Biol Year: 2022 Document type: Article Affiliation country: Estados Unidos Country of publication: Suiza