Short communication: Screening of proof-of-concept mutations of honey bee acetylcholinesterase 2 conferring resistance to organophosphorus and carbamate insecticides.
Comp Biochem Physiol C Toxicol Pharmacol
; 264: 109524, 2023 Feb.
Article
in En
| MEDLINE
| ID: mdl-36410640
ABSTRACT
The introduction of pesticide resistance-inducing mutations into target genes would in theory protect honey bees from the hazardous effects of pesticides. In this paper, to screen amino acid substitutions conferring resistance to organophosphorus and carbamate insecticides, honey bee acetylcholinesterase 2 (AmAChE2) variants with several mutations (V260L, A316S, G342A, G342V, F407Y, and G342V/F407Y) were generated and expressed in vitro using a baculovirus system. The inhibition constants of recombinant native and mutated AmAChE2s against six pesticides were measured. As a result, the A316S mutation was shown to induce high resistance without a catalytic efficiency change.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pesticides
/
Insecticides
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Animals
Language:
En
Journal:
Comp Biochem Physiol C Toxicol Pharmacol
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2023
Document type:
Article