Your browser doesn't support javascript.
loading
Open thoracic surgical implantation of cardiac pacemakers in rats.
Yin, Rose T; Chen, Sheena W; Benjamin Lee, K; Choi, Yeon Sik; Koo, Jahyun; Yang, Quansan; Napolitano, Michael A; Ausra, Jokubas; Holleran, Timothy J; Lapiano, Jessica B; Alex Waters, E; Brikha, Anlil; Kowalik, Grant; Miniovich, Alana N; Knight, Helen S; Russo, Bender A; Kiss, Alexi; Murillo-Berlioz, Alejandro; Efimova, Tatiana; Haney, Chad R; Gutruf, Philipp; Rogers, John A; Trachiotis, Gregory D; Efimov, Igor R.
Affiliation
  • Yin RT; Department of Biomedical Engineering, The George Washington University, Washington DC, USA.
  • Chen SW; Department of Surgery, The George Washington University, Washington DC, USA.
  • Benjamin Lee K; Department of Surgery, The George Washington University, Washington DC, USA.
  • Choi YS; Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA.
  • Koo J; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA.
  • Yang Q; Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA.
  • Napolitano MA; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA.
  • Ausra J; Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA.
  • Holleran TJ; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA.
  • Lapiano JB; Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA.
  • Alex Waters E; Department of Surgery, The George Washington University, Washington DC, USA.
  • Brikha A; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA.
  • Kowalik G; Department of General Surgery, Georgetown University, Washington DC, USA.
  • Miniovich AN; Department of General Surgery, Georgetown University, Washington DC, USA.
  • Knight HS; Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
  • Russo BA; Center for Advanced Molecular Imaging, Northwestern University, Evanston, IL, USA.
  • Kiss A; Center for Advanced Molecular Imaging, Northwestern University, Evanston, IL, USA.
  • Murillo-Berlioz A; Department of Biomedical Engineering, The George Washington University, Washington DC, USA.
  • Efimova T; Department of Biomedical Engineering, The George Washington University, Washington DC, USA.
  • Haney CR; Department of Biomedical Engineering, The George Washington University, Washington DC, USA.
  • Gutruf P; Department of Biomedical Engineering, The George Washington University, Washington DC, USA.
  • Rogers JA; Department of Anatomy and Cell Biology, The George Washington University, Washington DC, USA.
  • Trachiotis GD; The George Washington Cancer Center, The George Washington University, Washington DC, USA.
  • Efimov IR; Department of Cardiothoracic Surgery, Veteran Affairs Medical Center, Washington DC, USA.
Nat Protoc ; 18(2): 374-395, 2023 02.
Article in En | MEDLINE | ID: mdl-36411351
ABSTRACT
Genetic engineering and implantable bioelectronics have transformed investigations of cardiovascular physiology and disease. However, the two approaches have been difficult to combine in the same species genetic engineering is applied primarily in rodents, and implantable devices generally require larger animal models. We recently developed several miniature cardiac bioelectronic devices suitable for mice and rats to enable the advantages of molecular tools and implantable devices to be combined. Successful implementation of these device-enabled studies requires microsurgery approaches that reliably interface bioelectronics to the beating heart with minimal disruption to native physiology. Here we describe how to perform an open thoracic surgical technique for epicardial implantation of wireless cardiac pacemakers in adult rats that has lower mortality than transvenous implantation approaches. In addition, we provide the methodology for a full biocompatibility assessment of the physiological response to the implanted device. The surgical implantation procedure takes ~40 min for operators experienced in microsurgery to complete, and six to eight surgeries can be completed in 1 d. Implanted pacemakers provide programmed electrical stimulation for over 1 month. This protocol has broad applications to harness implantable bioelectronics to enable fully conscious in vivo studies of cardiovascular physiology in transgenic rodent disease models.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pacemaker, Artificial / Cardiac Surgical Procedures Limits: Animals Language: En Journal: Nat Protoc Year: 2023 Document type: Article Affiliation country: Estados Unidos
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pacemaker, Artificial / Cardiac Surgical Procedures Limits: Animals Language: En Journal: Nat Protoc Year: 2023 Document type: Article Affiliation country: Estados Unidos