DNAzyme-templated exponential isothermal amplification for sensitive detection of lead pollution and high-throughput screening of microbial biosorbents.
Sci Total Environ
; 863: 160899, 2023 Mar 10.
Article
in En
| MEDLINE
| ID: mdl-36521620
ABSTRACT
Very low concentrations of lead (Pb2+) pollution can have far-reaching adverse impacts on human health, due to the cumulative toxicity of Pb2+. Herein, we report a DNAzyme-templated exponential isothermal amplification strategy (termed DNAzymee) for the ultrasensitive detection of Pb2+ pollution and the high-throughput screening of microbial biosorbents to remove Pb2+ pollution. DNAzyme can specifically recognize Pb2+, and this recognition event can be amplified by the subsequent exponential isothermal amplification reaction (EXPAR) and monitored by a G-quadruplex specific dye. The proposed design showed a low limit of detection (95 pM) and could identify Pb2+ pollution in different real samples with high precision. In particular, the proposed assay was used to screen Pb2+ biosorbents, and the results showed that Leuconostoc mesenteroides is a promising microbial biosorbent for removing Pb2+ pollution. Thus, the DNAzymee assay can serve as a platform to monitor lead pollution in the environment and screen efficient biosorbents for the control of lead pollution.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biodegradation, Environmental
/
Biosensing Techniques
/
DNA, Catalytic
/
Environmental Pollutants
/
Environmental Pollution
/
Lead
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
En
Journal:
Sci Total Environ
Year:
2023
Document type:
Article
Affiliation country:
China
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS