Roles of Bothrops jararacussu toxins I and II: Antiviral findings against Zika virus.
Int J Biol Macromol
; 227: 630-640, 2023 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-36529220
ABSTRACT
Zika virus is the etiologic agent of Zika fever, and has been previously associated with cases of microcephaly, drawing the attention of the health authorities worldwide. However, no vaccine or antiviral are currently available. Phospholipases A2 (PLA2) isolated from snake venoms have demonstrated antiviral activity against several viruses. Here we demonstrated the anti-ZIKV activity of bothropstoxins-I and II (BthTX-I and II) isolated from Bothrops jararacussu venom. Vero E6 cells were infected with ZIKVPE243 in the presence of compounds for 72 h, when virus titers were evaluated. BthTX-I and II presented strong dose-dependent inhibition of ZIKV, with a SI of 149.1 and 1.44 × 105, respectively. These toxins mainly inhibited the early stages of the replicative cycle, such as during the entry of ZIKV into host cells, as shown by the potent virucidal effect, suggesting the action of these toxins on the virus particles. Moreover, BthTX-I and II presented significant activity towards post-entry stages of the ZIKV replicative cycle. Molecular docking analyses showed that BthTX-I and II potentially interact with DII and DIII domains from ZIKV Envelope protein. Our findings show that these PLA2s could be used as useful templates for the development of future antiviral candidate drugs against Zika fever.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bothrops
/
Crotalid Venoms
/
Zika Virus
/
Zika Virus Infection
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Int J Biol Macromol
Year:
2023
Document type:
Article
Affiliation country:
Brasil