Primer-Engineered Transferase Enzyme for One-Pot and Amplified Detection of Cobalt Pollution and Peptide Remover Screening.
J Agric Food Chem
; 71(1): 877-883, 2023 Jan 11.
Article
in En
| MEDLINE
| ID: mdl-36563311
ABSTRACT
Extensive consumption of cobalt in the chemical field such as for battery materials, alloy, pigments, and dyes has aggravated the pollution of cobalt both in food and the environment, and assays for its on-site monitoring are urgently demanded. Herein, we utilized enzyme dependence on metal cofactors to develop terminal transferase (TdT) as a recognition element, achieving a one-pot sensitive and specific assay for detecting cobalt pollution. We engineered a 3'-OH terminus primer to improve the discrimination capacity of TdT for Co2+ from other bivalent cations. The TdT extension reaction amplified the recognition of Co2+ and yielded a limit of detection of 0.99 µM for Co2+ detection. Then, the TdT-based assay was designed to precisely detect cobalt in food and agricultural soil samples. By end-measurement of fluorescence using a microplate reader, the multiplexing assay enabled the rapid screening of the peptide remover for cobalt pollution. The TdT-based assay can be a promising tool for cobalt pollution monitoring and control.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transferases
/
Cobalt
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
J Agric Food Chem
Year:
2023
Document type:
Article
Affiliation country:
China