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Antiandrogenic and Estrogenic Activity Evaluation of Oxygenated and Nitrated Polycyclic Aromatic Hydrocarbons Using Chemically Activated Luciferase Expression Assays.
Misaki, Kentaro; Tue, Nguyen Minh; Takamura-Enya, Takeji; Takigami, Hidetaka; Suzuki, Go; Tuyen, Le Huu; Takahashi, Shin; Tanabe, Shinsuke.
Affiliation
  • Misaki K; Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan.
  • Tue NM; School of Nursing, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
  • Takamura-Enya T; Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan.
  • Takigami H; Key Laboratory of Analytical Technology for Environmental Quality and Food Safety Control (KLATEFOS), VNU University of Science, Vietnam National University, 334 Nguyen Trai, Hanoi 11400, Vietnam.
  • Suzuki G; Department of Applied Chemistry, Kanagawa Institute of Technology, 1030 Shimo-Ogino, Atsugi 243-0292, Japan.
  • Tuyen LH; Center for Material Cycles and Waste Management Research, National Institute for Environmental Studies (NIES), Onogawa 16-2, Tsukuba 305-8506, Japan.
  • Takahashi S; Center for Material Cycles and Waste Management Research, National Institute for Environmental Studies (NIES), Onogawa 16-2, Tsukuba 305-8506, Japan.
  • Tanabe S; Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan.
Article in En | MEDLINE | ID: mdl-36612408
To establish the risk of the endocrine disrupting activity of polycyclic aromatic compounds, especially oxygenated and nitrated polycyclic aromatic hydrocarbons (oxy-PAHs and nitro-PAHs, respectively), antiandrogenic and estrogenic activities were determined using chemically activated luciferase expression (CALUX) assays with human osteoblast sarcoma cells. A total of 27 compounds including 9 oxy-PAHs (polycyclic aromatic ketones and quinones) and 8 nitro-PAHs was studied. The oxy-PAHs of 7H-benz[de]anthracen-7-one (BAO), 11H-benzo[a]fluoren-11-one (B[a]FO), 11H-benzo[b]fluoren-11-one (B[b]FO), and phenanthrenequinone (PhQ) exhibited significantly the potent inhibition of AR activation. All nitro-PAHs exhibited high antiandrogenic activities (especially high for 3-nitrofluoranthene (3-NFA) and 3-nitro-7H-benz[de]anthracen-7-one (3-NBAO)), and the AR inhibition was confirmed as noncompetitive for 3-NFA, 3-NBAO, and 1,3-dinitropyrene (1,3-DNPy). Antiandrogenic activity of 3-NFA demonstrated characteristically a U-shaped dose-response curve; however, the absence of fluorescence effect on the activity was confirmed. The prominent estrogenic activity dependent on dose-response curve was confirmed for 2 oxy-PAHs (i.e., B[a]FO and B[b]FO). Elucidating the role of AR and ER on the effects of polycyclic aromatic compounds (e.g., oxy- and nitro-PAHs) to endocrine dysfunctions in mammals and aquatic organisms remains a challenge.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polycyclic Compounds / Polycyclic Aromatic Hydrocarbons Limits: Animals / Humans Language: En Journal: Int J Environ Res Public Health Year: 2022 Document type: Article Affiliation country: Japón Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polycyclic Compounds / Polycyclic Aromatic Hydrocarbons Limits: Animals / Humans Language: En Journal: Int J Environ Res Public Health Year: 2022 Document type: Article Affiliation country: Japón Country of publication: Suiza