Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Cerebellum
; 23(1): 101-111, 2024 Feb.
Article
in En
| MEDLINE
| ID: mdl-36626013
ABSTRACT
Elucidation of the mechanisms involved in neurodegenerative diseases of the cerebellum has been hampered by the lack of robust single cell models to study Purkinje neurons and replicate at the same time in vivo features. Cerebellar Purkinje neurons are difficult to grow in dispersed cell culture, and only limited work has been done using rat cells. We developed a refined protocol for growing rat Purkinje neurons from embryonic and postnatal tissue ex vivo that results in well-developed, mature, functional, and synaptically active neurons. The rat Purkinje neurons generated are responsive to paracrine factors and genetic manipulation, allowing great experimental flexibility at the single-cell level. This ex vivo model can be used to investigate disease mechanisms that disturb Purkinje neuron morphology, function, and communication in high- and low-throughput screening formats.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Purkinje Cells
/
Cerebellum
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Cerebellum
Journal subject:
CEREBRO
Year:
2024
Document type:
Article
Affiliation country:
Noruega