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Label-free microfluidic device reveals single cell phagocytic activity and screens plant medicine rapidly.
Liu, Yameng; Wang, Miaomiao; Liu, Ruojin; Qiu, Feng.
Affiliation
  • Liu Y; School of Chinese Meteria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. fengqiu20070118@163.com.
  • Wang M; School of Chinese Meteria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. fengqiu20070118@163.com.
  • Liu R; School of Chinese Meteria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. fengqiu20070118@163.com.
  • Qiu F; School of Chinese Meteria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. fengqiu20070118@163.com.
Lab Chip ; 23(3): 553-559, 2023 01 31.
Article in En | MEDLINE | ID: mdl-36688537
Phagocytic activity is an extremely important indicator that evaluates medicinal effects related to the immune system and functions to investigate the mechanism of how a drug works under conditions such as immunological regulation, immune tolerance, inflammation, cancer, etc. Current techniques based on flow cytometry, fluorescence imaging or numbering CFUs after cell lysis for detecting phagocytosis suffer from long terms of bacteria culturing and complex preparation steps for fluorescent labeling or require a large amount of cell samples to be tested. This study aims at developing a simple and fast method for testing the phagocytic activity of unlabeled and native cells, taking advantage of very high-resolution direct current insulator-based dielectrophoresis (DC-iDEP). The properties of cells are characterized by native whole cell biophysical properties. This strategy not only eliminates the time-consuming bacterial culture work after cell lysis, but also lowers the expenses of bacteria labeling. The introduction of microfluidics reduces the sample volume or reagent needed. The analysis of the biophysical property distributions of native cells and medicine treated cells may lead to a less expensive and rapid tool for evaluating medicinal effects. Furthermore, berberine was investigated for decreasing the phagocytic activity of macrophages and used for comparison of activities. This study works on establishing a label-free, unbiased, and non-destructive method to determine cell phagocytic activity and investigate its use in evaluating medicinal effects on phagocytosis in a single step within a short time.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phagocytes / Microfluidics Language: En Journal: Lab Chip Journal subject: BIOTECNOLOGIA / QUIMICA Year: 2023 Document type: Article Affiliation country: China Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phagocytes / Microfluidics Language: En Journal: Lab Chip Journal subject: BIOTECNOLOGIA / QUIMICA Year: 2023 Document type: Article Affiliation country: China Country of publication: Reino Unido