Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation.
Chem Commun (Camb)
; 59(27): 4063-4066, 2023 Mar 30.
Article
in En
| MEDLINE
| ID: mdl-36938583
ABSTRACT
An efficient total chemical synthesis of site-specifically sumoylated histone H4 was undertaken to generate homogenously modified mononucleosomes. These were tested as substrates in biochemical assays with the histone H2B-specific ubiquitin ligases Rad6 and Bre1, which revealed the strong inhibition of H2B ubiquitylation by SUMO. This novel negative biochemical crosstalk between SUMO and ubiquitin was also confirmed to exist in human cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Histones
/
Saccharomyces cerevisiae Proteins
Limits:
Humans
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2023
Document type:
Article
Affiliation country:
Estados Unidos