Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming.
Cell Stem Cell
; 30(4): 450-459.e9, 2023 04 06.
Article
in En
| MEDLINE
| ID: mdl-36944335
ABSTRACT
We recently demonstrated the chemical reprogramming of human somatic cells to pluripotent stem cells (hCiPSCs), which provides a robust approach for cell fate manipulation. However, the utility of this chemical approach is currently hampered by slow kinetics. Here, by screening for small molecule boosters and systematically optimizing the original condition, we have established a robust, chemically defined reprogramming protocol, which greatly shortens the induction time from â¼50 days to a minimum of 16 days and enables highly reproducible and efficient generation of hCiPSCs from all 17 tested donors. We found that this optimized protocol enabled a more direct reprogramming process by promoting cell proliferation and oxidative phosphorylation metabolic activities at the early stage. Our results highlight a distinct chemical reprogramming pathway that leads to a shortcut for the generation of human pluripotent stem cells, which represents a powerful strategy for human cell fate manipulation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pluripotent Stem Cells
/
Induced Pluripotent Stem Cells
Limits:
Humans
Language:
En
Journal:
Cell Stem Cell
Year:
2023
Document type:
Article