Endoplasmic reticulum stress response and the regulation of endometrial interferon-beta production.
F S Sci
; 4(2): 151-162, 2023 05.
Article
in En
| MEDLINE
| ID: mdl-37011812
ABSTRACT
OBJECTIVE:
To gain an understanding of the potential role of endoplasmic reticulum (ER) stress in the endometrial compartment during early pregnancy, a highly understudied area.DESIGN:
This study examined the regulation of interferon-ß (IFNß) in response to ER stress in human decidualized and nondecidualized endometrial cells (human endometrial stromal cells [HESCs]) in vitro. In vivo, we examined ER stress and the IFNß levels locally in the mouse endometrium before and after implantation at embryonic day (E)1, E3, and E6.SETTING:
The study was performed in a reproductive sciences laboratory for Human Growth and Development. PATIENT(S) None. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) Quantitative polymerase chain reaction, Western blotting, and immunohistochemical analysis allowed us to test the action of endogenous ER stress activation in the endometrial compartment likely triggered by implantation and its ability to increase the endometrial IFNß levels. RESULT(S) In vitro, we observed a significant difference in the IFNß levels in HESCs, in response to ER stress activation, where decidualized HESCs exhibited a threefold increase in the IFNß levels compared with nondecidualized HESCs. Apoptotic caspase-3 activation was also isolated to the decidualized cells as a result of ER stress-dependent suppression of nuclear factor-kappa beta-regulated antiapoptotic factors, XIAP and MCL-1. In vivo, mouse endometrial IFNß was present in F4/80-positive macrophages at all time points examined. After implantation (E6), the mouse luminal epithelial cells robustly coexpressed both IFNß and the ER stress marker immunoglobulin heavy chain binding protein (BiP). CONCLUSION(S) These analyses demonstrate that both in vivo and in vitro, differentiated and decidualized endometrial cells undergoing ER stress have the capacity to produce increased IFNß levels; therefore, ER stress activation in the endometrial compartment may play a vital role in promoting successful implantation events.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Embryo Implantation
/
Endometrium
Limits:
Animals
/
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
F S Sci
Year:
2023
Document type:
Article