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Enhanced resistance to Bacillus thuringiensis Cry1Ac toxin mediated by the activation of prophenoloxidase in a cosmopolitan pest.
Li, Zeyun; Xiong, Lei; Li, Jingge; Yao, Shuyuan; Dong, Yi; Li, Yongbin; Chen, Xuanhao; Ye, Min; Zhang, Yangrui; Xie, Xuefeng; You, Minsheng; Yuchi, Zhiguang; Liu, Yuanyuan; You, Shijun.
Affiliation
  • Li Z; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Xiong L; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Li J; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Yao S; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Dong Y; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Li Y; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Chen X; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Ye M; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Zhang Y; BGI-Sanya, BGI-Shenzhen, Sanya 572025, China. Electronic address: zhangyangrui@genomics.cn.
  • Xie X; BGI-Sanya, BGI-Shenzhen, Sanya 572025, China. Electronic address: xiexuefeng@genomics.cn.
  • You M; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
  • Yuchi Z; School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. Electronic address: yuchi@tju.edu.cn.
  • Liu Y; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; BGI-Sanya, BGI-Shenzhen, Sanya 572025, China; Basic Forestry and Proteomics Center, Haixia Institute of Science and Technology, Fujian Agric
  • You S; State Key Laboratory of Crop Pest Control in Fujian and Taiwan, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzho
Int J Biol Macromol ; 242(Pt 1): 124678, 2023 Jul 01.
Article in En | MEDLINE | ID: mdl-37141972
Plutella xylostella has evolved resistance to Bacillus thuringiensis Cry1Ac toxin over a long evolutionary period. Enhanced immune response is an important factor in insect resistance to a variety of insecticides, and whether phenoloxidase (PO), an immune protein, is involved in resistance to Cry1Ac toxin in P. xylostella remains unclear. Here, spatial and temporal expression patterns showed that prophenoloxidase (PxPPO1 and PxPPO2) in the Cry1S1000-resistant strain was more highly expressed in eggs, 4th instar, head, and hemolymph than those in G88-susceptible strain. The results of PO activity analysis showed that after treatment with Cry1Ac toxin PO activity was about 3 times higher than that before treatment. Furthermore, knockout of PxPPO1 and PxPPO2 significantly increased the susceptibility to Cry1Ac toxin. These findings were further supported by the knockdown of Clip-SPH2, a negative regulator of PO, which resulted in increased PxPPO1 and PxPPO2 expression and Cry1Ac susceptibility in the Cry1S1000-resistant strain. Finally, the synergistic effect of quercetin showed that larval survival decreased from 100 % to <20 % compared to the control group. This study will provide a theoretical basis for the analysis of immune-related genes (PO) genes involved in the resistance mechanism and pest control of P. xylostella.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus thuringiensis / Moths Limits: Animals Language: En Journal: Int J Biol Macromol Year: 2023 Document type: Article Country of publication: Países Bajos
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus thuringiensis / Moths Limits: Animals Language: En Journal: Int J Biol Macromol Year: 2023 Document type: Article Country of publication: Países Bajos