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Construction, expression and assemble of EMCV VLPs and their potency evaluation.
Zhang, Haixia; Li, Shengjun; Su, Jinxian; Ma, Xiaomei; Ali, Amjad; Xie, Jingying; Ma, Zhongren; Feng, Ruofei.
Affiliation
  • Zhang H; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China; Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China.
  • Li S; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China.
  • Su J; College of Life Science and Engineering, Northwest Minzu University, Lanzhou, 730030, China.
  • Ma X; College of Life Science and Engineering, Northwest Minzu University, Lanzhou, 730030, China.
  • Ali A; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China.
  • Xie J; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China; College of Life Science and Engineering, Northwest Minzu University, Lanzhou, 730030, China.
  • Ma Z; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China; Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China.
  • Feng R; Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China; Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China. Electron
Virology ; 584: 1-8, 2023 07.
Article in En | MEDLINE | ID: mdl-37167793
ABSTRACT
Encephalomycarditis virus (EMCV) is an essential pathogen with a broad host range and causes enormous economic losses to the pig industry worldwide. Here, we constructed and assembled the EMCV virus-like particles (VLPs) in vitro and verified high efficiency of virus protection. Results showed that the proteins auto-assembled into VLPs successfully in vitro. The animal experiments revealed that high-titer antibody production is triggered by VLPs. Meanwhile, the mice challenged with EMCV were obviously protected. The protection rate of group VLPs with the adjuvant was 75%, while that of the VLPs group was 62.5% compared to the control. These findings indicate that recombinant EMCV VLPs have a remarkable anti-EMCV effect and could be a new vaccine candidate for the control of EMCV infection.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vaccines, Virus-Like Particle / Antibodies, Viral Limits: Animals Language: En Journal: Virology Year: 2023 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vaccines, Virus-Like Particle / Antibodies, Viral Limits: Animals Language: En Journal: Virology Year: 2023 Document type: Article Affiliation country: China