Effect of elevated irrigation bottle height during cataract surgery on corneal endothelial cells in porcine eyes.
BMC Ophthalmol
; 23(1): 211, 2023 May 11.
Article
in En
| MEDLINE
| ID: mdl-37170242
ABSTRACT
BACKGROUND:
Cataract surgery induces corneal endothelial cell loss (ECL). This study investigates the relationship between bottle height (BH) and ECL induced due to irrigation and aspiration (I/A) in cataract surgery and quantifies protective effects of intraoperatively used ophthalmic viscoelastic substances.METHODS:
Intermittent I/A without phacoemulsification was performed in porcine eyes for 10 min with varying BHs of 100 cm (BH100), 125 cm (BH125), 150 cm (BH150) or no treatment (control, no I/A). Additionally, in one group a dispersive ophthalmic viscoelastic substance was injected into the anterior eye chamber before treatment with I/A at a BH of 150 cm (BH150 + V). After exposure of the corneal endothelium to I/A, the corneas were prepared to split corneal buttons on day 0 and cultivated for 15 days. Endothelial cell density (ECD) was analyzed blinded on days 1, 8 and 15.RESULTS:
Relative ECL significantly correlated with irrigation BH (control (n = 13) -9.69 ± 6.03% (average ± standard deviation); BH100 (n = 12) -9.69 ± 4.81%-p = 1.000; BH125 (n = 14) -19.44 ± 7.30% - p < 0.001; BH150 (n = 13) -21.99 ± 6.70%-p < 0.001). I/A-induced ECL was significantly decreased by the injection of ophthalmic viscoelastic, as BH150 + V (n = 14; -10.92 ± 4.09%-p = 1.000) showed a cell loss comparable to the control group.CONCLUSIONS:
ECL is altered by I/A BH and reduced when viscoelastic substances are used.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cataract
/
Cataract Extraction
/
Phacoemulsification
Limits:
Animals
Language:
En
Journal:
BMC Ophthalmol
Journal subject:
OFTALMOLOGIA
Year:
2023
Document type:
Article
Affiliation country:
Alemania