Applications and Tuning Strategies for Transcription Factor-Based Metabolite Biosensors.
Biosensors (Basel)
; 13(4)2023 Mar 28.
Article
in En
| MEDLINE
| ID: mdl-37185503
ABSTRACT
Transcription factor (TF)-based biosensors are widely used for the detection of metabolites and the regulation of cellular pathways in response to metabolites. Several challenges hinder the direct application of TF-based sensors to new hosts or metabolic pathways, which often requires extensive tuning to achieve the optimal performance. These tuning strategies can involve transcriptional or translational control depending on the parameter of interest. In this review, we highlight recent strategies for engineering TF-based biosensors to obtain the desired performance and discuss additional design considerations that may influence a biosensor's performance. We also examine applications of these sensors and suggest important areas for further work to continue the advancement of small-molecule biosensors.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Biosensing Techniques
Language:
En
Journal:
Biosensors (Basel)
Year:
2023
Document type:
Article
Affiliation country:
Estados Unidos