Automated identification of single and clustered chromosomes for metaphase image analysis.

ABSTRACT

Background: Chromosome analysis is laborious and time-consuming. Automated methods can significantly increase the efficiency of chromosome analysis. For the automated analysis of chromosome images, single and clustered chromosomes must be identified. Herein, we propose a feature-based method for distinguishing between single chromosomes and clustered chromosome. Method: The proposed method comprises three main steps. In the first step, chromosome objects are segmented from metaphase chromosome images in advance. In the second step, seven features are extracted from each segmented object, i.e., the normalized area, area/boundary ratio, side branch index, exhaustive thresholding index, normalized minimum width, minimum concave angle, and maximum boundary shift. Finally, the segmented objects are classified as a single chromosome or chromosome cluster using a combination of the seven features. Results: In total, 43,391 segmented objects, including 39,892 single chromosomes and 3,499 chromosome clusters, are used to evaluate the proposed method. The results show that the proposed method achieves an accuracy of 98.92% by combining the seven features using support vector machine. Conclusions: The proposed method is highly effective in distinguishing between single and clustered chromosomes and can be used as a preprocessing procedure for automated chromosome image analysis.