Intra-stromal injection of honey-treated keratocytes as a cell-based therapy for experimental corneal laceration.
J Complement Integr Med
; 20(3): 604-611, 2023 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-37277938
ABSTRACT
OBJECTIVES:
This study aimed to investigate the potential of honey-supplemented medium (HSM) for expanding corneal keratocytes and its transplantation in a model of corneal laceration.METHODS:
Keratocytes were cultured in 1â¯% HSM- or 10â¯% fetal bovine serum (FBS)-supplemented medium for 24â¯h. The effect of HSM on keratocyte proliferation was evaluated using the MTT assay. The relative expression of Lum, Kera, and ALDH3A1, known markers of native keratocytes, was quantified by real-time PCR. The safety and efficacy of HSM-treated keratocyte intrastromal injection in a rabbit model of corneal laceration were also evaluated.RESULTS:
The MTT assay showed that HSM treatment did not significantly affect cell viability compared to FBS-supplemented medium (84.71 ± 2.38 vs. 100.08 ± 10.92, respectively; p=0.076). Moreover, HSM-treated keratocytes had significantly increased expression of Lum, Kera, and ALDH3A1 compared to cells treated with FBS, while the expression of the proliferation biomarker Thy-1 did not significantly differ between the two treatments. Intrastromal injection of HSM-treated keratocytes in the laceration animal model was safe and uneventful, resulting in less stromal inflammation and neovascularization, and consequently, better final architecture with less residual haze compared to the group injected with FBS-treated keratocytes.CONCLUSIONS:
These findings suggest that honey is a suitable supplement for keratocyte treatment and corneal cell therapy. The use of HSM may have potential applications in the treatment of corneal injuries and diseases.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lacerations
/
Corneal Injuries
/
Honey
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Complement Integr Med
Year:
2023
Document type:
Article
Affiliation country:
Irán