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Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon.
Gomes, Erika Oliveira; Sacchetto, Lívia; Teixeira, Maurício; Chaves, Bárbara Aparecida; Hendy, Adam; Mendonça, Claudia; Guimarães, Izabele; Linhares, Ramon; Brito, Daniela; Valério, Danielle; Cordeiro, Jady Shayenne Mota; Neto, Alexandre Vilhena Silva; Sampaio, Vanderson Souza; Scarpassa, Vera Margarete; Buenemann, Michaela; Vasilakis, Nikos; Baia-da-Silva, Djane Clarys; Nogueira, Maurício Lacerda; Mourão, Maria Paula Gomes; Lacerda, Marcus Vinícius Guimarães.
Affiliation
  • Gomes EO; Universidade do Estado do Amazonas (UEA), Manaus 69850-000, AM, Brazil.
  • Sacchetto L; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Teixeira M; Laboratório de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus 69067-375, AM, Brazil.
  • Chaves BA; Laboratório de Pesquisa em Virologia, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, SP, Brazil.
  • Hendy A; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Mendonça C; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Guimarães I; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Linhares R; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Brito D; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Valério D; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Cordeiro JSM; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Neto AVS; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Sampaio VS; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Scarpassa VM; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Buenemann M; Programa de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
  • Vasilakis N; Instituto Todos pela Saúde (ITpS), São Paulo 01310-942, SP, Brazil.
  • Baia-da-Silva DC; Laboratório de Genética Populacional e Evolução de Mosquitos Vetores da Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus 69067-375, AM, Brazil.
  • Nogueira ML; Department of Geography, New Mexico State University, Las Cruces, NM 88003, USA.
  • Mourão MPG; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Lacerda MVG; Center for Vector-Borne and Zoonotic Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
Viruses ; 15(6)2023 06 12.
Article in En | MEDLINE | ID: mdl-37376655
ABSTRACT
Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Aedes / Zika Virus / Zika Virus Infection Type of study: Diagnostic_studies Limits: Animals / Female / Humans Country/Region as subject: America do sul / Brasil Language: En Journal: Viruses Year: 2023 Document type: Article Affiliation country: Brasil
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Aedes / Zika Virus / Zika Virus Infection Type of study: Diagnostic_studies Limits: Animals / Female / Humans Country/Region as subject: America do sul / Brasil Language: En Journal: Viruses Year: 2023 Document type: Article Affiliation country: Brasil