Effects of mesenchymal stem cells on Treg cells in rats with colitis.
Clin Exp Immunol
; 214(3): 296-303, 2023 12 13.
Article
in En
| MEDLINE
| ID: mdl-37417713
ABSTRACT
The aim was to investigate the therapeutic effect of bone marrow mesenchymal stem cells (BM-MSC) on dextran sulfate sodium (DSS) induced colitis in rats and its effect on regulatory T cells (Treg). A model of DSS-induced colitis was established. BM-MSC was isolated and cultured to observe the efficacy of BM-MSC on colitis, including general vital signs, weight changes, colonic length changes, colonic histopathological changes, and colonic tissue MPO activity. The expression of inflammatory factors (IFN-γ, IL-4, IL-17, TGF-ß) in colonic tissues was measured by real-time PCR. The amount of CD4â
+â
CD25â
+â
Treg was detected by flow cytometry. Real-time PCR was used to detect Foxp3+mRNA in CD4â
+â
CD25â
+â
Treg, western to detect Foxp3+protein expression in CD4â
+â
CD25â
+â
Treg, and ELISA was used to detect IL-35 and IL-10 cytokines in CD4â
+â
CD25â
+â
Treg culture supernatant. Results show that intravenous injection of BM-MSC significantly improved the clinical manifestations and histopathological changes in rats with experimental DSS colitis; significantly down-regulated the expression of inflammatory factors IFN-γ, IL-4, and IL-17 and up-regulated the expression of TGF-ß in colon tissues; BM-MSC also increased the number of CD4+CD25+Foxp3+Treg and enhanced the function of CD4+CD25+Foxp3+Treg in colon tissues, and up-regulated the expression of IL-35. In conclusion, BM-MSC has a certain therapeutic effect on DSS-induced colitis. It can improve the general signs of colitis rats and reduce intestinal injury and inflammatory response. The immunoregulatory effect of BM-MSC is achieved by enhancing the function of CD4+CD25+Foxp3+Treg and up-regulating the secretion of immunosuppressive inflammatory factors.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Colitis
/
Mesenchymal Stem Cells
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Clin Exp Immunol
Year:
2023
Document type:
Article
Affiliation country:
China