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Scale-down of CHO cell cultivation from shake flasks based on oxygen mass transfer allows application of parallelized, non-invasive, and time-resolved monitoring of the oxygen transfer rate in 48-well microtiter plates.
Ihling, Nina; Berg, Christoph; Paul, Richard; Munkler, Lara Pauline; Mäkinen, Meeri E-L; Chotteau, Veronique; Büchs, Jochen.
Affiliation
  • Ihling N; AVT - Biochemical Engineering, RWTH Aachen University, Aachen, Germany.
  • Berg C; AVT - Biochemical Engineering, RWTH Aachen University, Aachen, Germany.
  • Paul R; AVT - Biochemical Engineering, RWTH Aachen University, Aachen, Germany.
  • Munkler LP; AVT - Biochemical Engineering, RWTH Aachen University, Aachen, Germany.
  • Mäkinen ME; KTH Royal Institute of Technology, Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden.
  • Chotteau V; KTH Royal Institute of Technology, Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden.
  • Büchs J; AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing, KTH, Stockholm, Sweden.
Biotechnol J ; 18(11): e2300053, 2023 Nov.
Article in En | MEDLINE | ID: mdl-37424196
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxygen / Cell Culture Techniques Limits: Animals Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2023 Document type: Article Affiliation country: Alemania Country of publication: Alemania
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxygen / Cell Culture Techniques Limits: Animals Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2023 Document type: Article Affiliation country: Alemania Country of publication: Alemania