Synergistic catalysis and detection of hydrogen peroxide based on a 3D-dimensional molybdenum disulfide interspersed carbon nanotubes nanonetwork immobilized chloroperoxidase biosensor.
Bioelectrochemistry
; 154: 108507, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-37451043
ABSTRACT
Enzyme-based electrochemical biosensors are promising for a wide range of applications due to their unique specificity and high sensitivity. In this work, we present a novel enzyme bioelectrode for the sensing of hydrogen peroxide (H2O2). The molybdenum disulfide nanoflowers (MoS2) is self-assembled on carboxylated carbon nanotubes (CNT) to form a three-dimensional conductive network (3D-CNT@MoS2), which is modified with 1-ethyl-3-methylimidazolium bromide (ILEMB), and followed by anchoring chloroperoxidase (CPO) onto the nanocomposite (3D-CNT@MoS2/ILEMB) through covalent binding to form a bioconjugate (3D-CNT@MoS2/ILEMB/CPO). The ILEMB modified 3D-CNT@MoS2/ILEMB has good hydrophilicity and conductivity, which not only provides a suitable microenvironment for the immobilization of CPO but also facilitates the direct electron transfer (DET) of CPO at the electrode. The 3D-CNT@MoS2/ILEMB/CPO bioconjugate modified electrode has a high catalytic efficiency for H2O2. Through electroenzymatic synergistic catalysis for H2O2 detection by 3D-CNT@MoS2/ILEMB/CPO-GCE, a wide detection range of 0.2 µmol·L-1 to 997 µmol·L-1 and a low detection limit of 0.097 µmolï½¥L-1 with high sensitivity of 1050 µA·mmol·L-1·cm-2 were achieved. Additionally, the 3D-CNT@MoS2/ILEMB/CPO-GCE displayed exceptional stability, selectivity, and reproducibility. Furthermore, 3D-CNT@MoS2/ILEMB/CPO-GCE is suitable for sensing of H2O2 in human urine s with good recovery, suggesting its potential application for the detection of H2O2 in biomedical field.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Chloride Peroxidase
/
Nanotubes, Carbon
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Bioelectrochemistry
Journal subject:
BIOQUIMICA
Year:
2023
Document type:
Article