Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC).

Loughran, Sinéad T; Bree, Ronan T; Walls, Dermot

Loughran, Sinéad T; Bree, Ronan T; Walls, Dermot.

Affiliation

Loughran ST; Department of Life and Health Sciences, School of Health and Science, Dundalk Institute of Technology, Dundalk, Louth, Ireland. sinead.loughran@dkit.ie.
Bree RT; Department of Life and Health Sciences, School of Health and Science, Dundalk Institute of Technology, Dundalk, Louth, Ireland.
Walls D; School of Biotechnology, Dublin City University, Dublin, Ireland.

Methods Mol Biol ; 2699: 193-223, 2023.

Article in En | MEDLINE | ID: mdl-37647000

Subject(s)
Key words

6xHistidine; Affinity chromatography; IMAC; Ni-NTA; Polyhistidine; Protein purification

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin Neoplasms / DNA, Recombinant Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2023 Document type: Article Affiliation country: Irlanda

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