[Danshenxinkun B protects human umbilical vein endothelial cells against ox-LDL-induced injury by inhibiting pyroptosis and the NF-κB/NLRP3 pathway].
Nan Fang Yi Ke Da Xue Xue Bao
; 43(8): 1425-1431, 2023 Aug 20.
Article
in Zh
| MEDLINE
| ID: mdl-37712281
ABSTRACT
OBJECTIVE:
To investigate the protective effect of Danshenxinkun B against oxidized low-density lipoprotein (ox-LDL)- induced human umbilical vein endothelial cell (HUVEC) injury and explore the underlying mechanism.METHODS:
HUVECs cultured in the presence of 10% fetal bovine serum were treated with ox-LDL (100 µg/mL), ox-LDL+0.1% dimethyl sulfoxide (DMSO), or ox-LDL+Danshenxinkun B (100 ng/mL, dissolved in DMSO) for 24 h. The changes in lactate dehydrogenase (LDH) release was detected, and qRT-PCR was used to detect the mRNA expressions of nuclear factor-κB1 (NF-κB1), nucleotide binding oligomerization domain-like receptor family pyrin domain protein 3 (NLRP3), gasdermin D (GSDMD) and interleukin- 1ß (IL-1ß). The protein expressions of NF-κB1, NLRP3, caspase-1, IL-1ß and GSDMD-N were detected with Western blotting. Immunofluorescence assay was performed to examine the changes in GSDMD expression in the cells.RESULTS:
Compared with the normal control cells, the cells treated with ox-LDL alone or in combination with DMSO exhibited significantly increased LDH release, mRNA expressions of NF-κB1, NLRP3, GSDMD, and IL- 1ß and the protein levels of NF-κB1, NLRP3, IL- 1ß, GSDMD-N and caspase-1 (P<0.01), which were all significantly lowered by treatment with Danshenxinkun B (P<0.05 or 0.01). Danshenxinkun B treatment significantly inhibited GSDMD expression on the cell membrane and restricted its entry into the cell nucleus.CONCLUSION:
Danshenxinkun B alleviates ox-LDL-induced HUVEC injury possibly by suppressing pyroptosis mediated by NLRP3 inflammatory bodies and inhibiting the NF-κB/NLRP3 signaling pathway.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
NF-kappa B
/
NLR Family, Pyrin Domain-Containing 3 Protein
Limits:
Humans
Language:
Zh
Journal:
Nan Fang Yi Ke Da Xue Xue Bao
Year:
2023
Document type:
Article
Affiliation country:
China