Network pharmacology and experimental verification reveal the mechanism of safranal against glioblastoma (GBM).
Front Oncol
; 13: 1255164, 2023.
Article
in En
| MEDLINE
| ID: mdl-37736545
ABSTRACT
Introduction:
Safranal is an active component of the traditional Tibetan medicine (TTM) saffron, which has potential anticancer activity. Methods andresults:
Here, we studied the therapeutic effect and mechanism of safranal on GBM. CCK-8, GBM-brain organoid coculture experiments and 3D tumour spheroid invasion assays showed that safranal inhibited GBM cell proliferation and invasion in vitro. Network pharmacology, RNA-seq, molecular docking analysis, western blotting, apoptosis, and cell cycle assays predicted and verified that safranal could promote GBM cell apoptosis and G2/M phase arrest and inhibit the PI3K/AKT/mTOR axis. In vivo experiments showed that safranal could inhibit GBM cell growth alone and in combination with TMZ.Conclusion:
This study revealed that safranal inhibits GBM cell growth in vivo and in vitro, promotes GBM cell apoptosis and G2/M phase arrest, inhibits the PI3K/AKT/mTOR axis and cooperate with TMZ.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Front Oncol
Year:
2023
Document type:
Article
Affiliation country:
China