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Expression, Intracellular Localization, and Maturation of Cysteine Cathepsins in Renal Embryonic and Cancer Cell Lines.
Frolova, Anastasia S; Tikhomirova, Natalia K; Kireev, Igor I; Zernii, Evgeni Yu; Parodi, Alessandro; Ivanov, Konstantin I; Zamyatnin, Andrey A.
Affiliation
  • Frolova AS; Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, 119991, Russia.
  • Tikhomirova NK; Research Center for Translational Medicine, Sirius University of Science and Technology, Sochi, 354340, Russia.
  • Kireev II; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
  • Zernii EY; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
  • Parodi A; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
  • Ivanov KI; Research Center for Translational Medicine, Sirius University of Science and Technology, Sochi, 354340, Russia.
  • Zamyatnin AA; Research Center for Translational Medicine, Sirius University of Science and Technology, Sochi, 354340, Russia.
Biochemistry (Mosc) ; 88(7): 1034-1044, 2023 Jul.
Article in En | MEDLINE | ID: mdl-37751872
ABSTRACT
Cysteine cathepsins play an important role in tumor development and metastasis. The expression of these enzymes is often increased in many types of tumor cells. Cysteine cathepsins contribute to carcinogenesis through a number of mechanisms, including proteolysis of extracellular matrix and signaling molecules on the cell surface, as well as degradation of transcription factors and disruption of signaling cascades in the cell nucleus. Distinct oncogenic functions have been reported for several members of the cysteine cathepsin family in various types of cancer, but a comparative study of all eleven cysteine cathepsins in one experimental model is still missing. In this work, we assessed and compared the expression, localization, and maturation of all eleven cysteine cathepsins in embryonic kidney cells HEK293 and kidney cancer cell lines 769-P and A-498. We found that the expression of cathepsins V, B, Z, L, and S was 3- to 9-fold higher in kidney tumor cells than in embryonic cells. We also showed that all cysteine cathepsins were present in varying amounts in the nucleus of both embryonic and tumor cells. Notably, more than half of the cathepsin Z or K and over 88% of cathepsin F were localized in tumor cell nuclei. Moreover, mature forms of cysteine cathepsins were more prevalent in tumor cells than in embryonic cells. These results can be further used to develop novel diagnostic tools and may assist in the investigation of cysteine cathepsins as potential therapeutic targets.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Biochemistry (Mosc) Year: 2023 Document type: Article Affiliation country: Rusia

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Biochemistry (Mosc) Year: 2023 Document type: Article Affiliation country: Rusia