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Progenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction.
Liu, Qianglin; Li, Chaoyang; Li, Yuxia; Wang, Leshan; Zhang, Xujia; Deng, Buhao; Gao, Peidong; Shiri, Mohammad; Alkaifi, Fozi; Zhao, Junxing; Stephens, Jacqueline M; Simintiras, Constantine A; Francis, Joseph; Sun, Jiangwen; Fu, Xing.
Affiliation
  • Liu Q; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Li C; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Li Y; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Wang L; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Zhang X; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Deng B; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
  • Gao P; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Shiri M; Department of Computer Science, Old Dominion University, Norfolk, VA, USA.
  • Alkaifi F; Department of Computer Science, Old Dominion University, Norfolk, VA, USA.
  • Zhao J; Department of Animal Sciences, Shanxi Agricultural University, Taigu, Shanxi, China.
  • Stephens JM; Pennington Biomedical Research Center, Baton Rouge, LA, USA; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA.
  • Simintiras CA; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA.
  • Francis J; Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
  • Sun J; Department of Computer Science, Old Dominion University, Norfolk, VA, USA.
  • Fu X; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA, USA; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, USA. Electronic address: xfu1@agcenter.lsu.edu.
STAR Protoc ; 4(4): 102703, 2023 Dec 15.
Article in En | MEDLINE | ID: mdl-37948186
ABSTRACT
Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Adipose Tissue Limits: Animals Language: En Journal: STAR Protoc Year: 2023 Document type: Article Affiliation country: Estados Unidos
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Adipose Tissue Limits: Animals Language: En Journal: STAR Protoc Year: 2023 Document type: Article Affiliation country: Estados Unidos