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Impact of volume and expression time in an AAV-delivered channelrhodopsin.
Ansarifar, Sanaz; Andreike, Gabija; Nazari, Milad; Labouriau, Rodrigo; Nabavi, Sadegh; Moreno, Andrea.
Affiliation
  • Ansarifar S; Danish Institute of Translational Neuroscience (DANDRITE), Aarhus University, Aarhus, Denmark.
  • Andreike G; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
  • Nazari M; Danish Institute of Translational Neuroscience (DANDRITE), Aarhus University, Aarhus, Denmark.
  • Labouriau R; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
  • Nabavi S; Danish Institute of Translational Neuroscience (DANDRITE), Aarhus University, Aarhus, Denmark.
  • Moreno A; Centre for Proteins in Memory (PROMEMO), Danish National Research Foundation, Aarhus University, Aarhus, Denmark.
Mol Brain ; 16(1): 77, 2023 Nov 10.
Article in En | MEDLINE | ID: mdl-37950268
Optogenetics has revolutionised neuroscience research, but at the same time has brought a plethora of new variables to consider when designing an experiment with AAV-based targeted gene delivery. Some concerns have been raised regarding the impact of AAV injection volume and expression time in relation to longitudinal experimental designs. In this study, we investigated the efficiency of optically evoked post-synaptic responses in connection to two variables: the volume of the injected virus and the expression time of the virus. For this purpose, we expressed the blue-shifted ChR2, oChIEF, employing a widely used AAV vector delivery strategy. We found that the volume of the injected virus has a minimal impact on the efficiency of optically-evoked postsynaptic population responses. The expression time, on the other hand, has a pronounced effect, with a gradual reduction in the population responses beyond 4 weeks of expression. We strongly advise to monitor time-dependent expression profiles when planning or conducting long-term experiments that depend on successful and stable channelrhodopsin expression.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genetic Therapy / Genetic Vectors Language: En Journal: Mol Brain Journal subject: BIOLOGIA MOLECULAR / CEREBRO Year: 2023 Document type: Article Affiliation country: Dinamarca Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genetic Therapy / Genetic Vectors Language: En Journal: Mol Brain Journal subject: BIOLOGIA MOLECULAR / CEREBRO Year: 2023 Document type: Article Affiliation country: Dinamarca Country of publication: Reino Unido