Impact of volume and expression time in an AAV-delivered channelrhodopsin.
Mol Brain
; 16(1): 77, 2023 Nov 10.
Article
in En
| MEDLINE
| ID: mdl-37950268
Optogenetics has revolutionised neuroscience research, but at the same time has brought a plethora of new variables to consider when designing an experiment with AAV-based targeted gene delivery. Some concerns have been raised regarding the impact of AAV injection volume and expression time in relation to longitudinal experimental designs. In this study, we investigated the efficiency of optically evoked post-synaptic responses in connection to two variables: the volume of the injected virus and the expression time of the virus. For this purpose, we expressed the blue-shifted ChR2, oChIEF, employing a widely used AAV vector delivery strategy. We found that the volume of the injected virus has a minimal impact on the efficiency of optically-evoked postsynaptic population responses. The expression time, on the other hand, has a pronounced effect, with a gradual reduction in the population responses beyond 4 weeks of expression. We strongly advise to monitor time-dependent expression profiles when planning or conducting long-term experiments that depend on successful and stable channelrhodopsin expression.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Therapy
/
Genetic Vectors
Language:
En
Journal:
Mol Brain
Journal subject:
BIOLOGIA MOLECULAR
/
CEREBRO
Year:
2023
Document type:
Article
Affiliation country:
Dinamarca
Country of publication:
Reino Unido