The power of the QUENCHER method in measuring total antioxidant capacity of foods: Importance of interactions between different forms of antioxidants.

ABSTRACT

Antioxidants play a crucial role in maintaining human health by counteracting oxidative stress and regulating redox balance within the body. The mixture of various antioxidant compounds in different forms (i.e., free, bound, insoluble) in food creates a redox active environment both in the human body and in the food system. Acting as both electron donors and acceptors while interacting with each other can either result in antagonism through pro-oxidative effects, or synergism through regeneration of one antioxidant by another. During the antioxidant capacity measurement, besides the individual antioxidant effects of the antioxidant components, these effects that occur because of their interaction with each other should be also considered. Classical antioxidant capacity measurement methods mostly concentrate on the fractions of foods that can be extracted with either water, alcohol, lipid, or acid/alkaline solutions. Antioxidants that cannot be extracted with any solvent are mostly ignored in these methods. On the other hand, the QUENCHER method, which allows direct measurement of antioxidant capacity foods without extraction, offers a rational solution to the limitations of traditional extraction-based methods. This approach considers the antioxidant capacity and interactions of all antioxidant forms that can be found in a food matrix, at the same time. This review provides detailed insights into the advantages of QUENCHER as a holistic approach for the accurate measurement of the antioxidant capacity of foods.