Genome-wide screen reveals cellular functions that counteract rifampicin lethality in Escherichia coli.
Microbiol Spectr
; 12(1): e0289523, 2024 Jan 11.
Article
in En
| MEDLINE
| ID: mdl-38054714
ABSTRACT
IMPORTANCE Rifamycins are a group of antibiotics with a wide antibacterial spectrum. Although the binding target of rifamycin has been well characterized, the mechanisms underlying the discrepant killing efficacy between gram-negative and gram-positive bacteria remain poorly understood. Using a high-throughput screen combined with targeted gene knockouts in the gram-negative model organism Escherichia coli, we established that rifampicin efficacy is strongly dependent on several cellular pathways, including iron acquisition, DNA repair, aerobic respiration, and carbon metabolism. In addition, we provide evidence that these pathways modulate rifampicin efficacy in a manner distinct from redox-related killing. Our findings provide insights into the mechanism of rifamycin efficacy and may aid in the development of new antimicrobial adjuvants.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rifampin
/
Rifamycins
Language:
En
Journal:
Microbiol Spectr
Year:
2024
Document type:
Article
Affiliation country:
China