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Characterization of outer membrane vesicles released by clinical isolates of Neisseria gonorrhoeae.
Dhital, Subhash; Deo, Pankaj; Stuart, Isabella; Huang, Cheng; Zavan, Lauren; Han, Mei-Ling; Kaparakis-Liaskos, Maria; Ramm, Georg; Schittenhelm, Ralf B; Howden, Benjamin; Naderer, Thomas.
Affiliation
  • Dhital S; Department of Biochemistry & Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Deo P; Department of Biochemistry & Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Stuart I; Department of Biochemistry & Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Huang C; Department of Biochemistry & Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Zavan L; Monash Proteomics and Metabolomics Platform, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Han ML; Department of Microbiology, Anatomy, Physiology, and Pharmacology, La Trobe University, Melbourne, Victoria, Australia.
  • Kaparakis-Liaskos M; Research Centre for Extracellular Vesicles, School of Molecular Science, La Trobe University, Melbourne, Victoria, Australia.
  • Ramm G; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Schittenhelm RB; Department of Microbiology, Anatomy, Physiology, and Pharmacology, La Trobe University, Melbourne, Victoria, Australia.
  • Howden B; Research Centre for Extracellular Vesicles, School of Molecular Science, La Trobe University, Melbourne, Victoria, Australia.
  • Naderer T; Department of Biochemistry & Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Proteomics ; : e2300087, 2023 Dec 07.
Article in En | MEDLINE | ID: mdl-38059892
ABSTRACT
The sexually transmitted pathogen Neisseria gonorrhoeae releases membrane vesicles including outer membrane vesicles (OMVs) during infections. OMVs traffic outer membrane molecules, such as the porin PorB and lipo-oligosaccharide (LOS), into host innate immune cells, eliciting programmed cell death pathways, and inflammation. Little is known, however, about the proteome and LOS content of OMVs released by clinical strains isolated from different infection sites, and whether these vesicles similarly activate immune responses. Here, we characterized OMVs from four N. gonorrhoeae isolates and determined their size, abundance, proteome, LOS content, and activation of inflammatory responses in macrophages. The overall proteome of the OMVs was conserved between the four different isolates, which included major outer membrane and periplasm proteins. Despite this, we observed differences in the rate of OMV biogenesis and the relative abundance of membrane proteins and LOS. Consequently, OMVs from clinical isolates induced varying rates of macrophage cell death and the secretion of interleukin-1 family members, such as IL-1α and IL-1ß. Overall, these findings demonstrate that clinical isolates of N. gonorrhoeae utilize membrane vesicles to release proteins and lipids, which affects innate immune responses.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Proteomics Journal subject: BIOQUIMICA Year: 2023 Document type: Article Affiliation country: Australia

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Proteomics Journal subject: BIOQUIMICA Year: 2023 Document type: Article Affiliation country: Australia
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