Organization of an Artificial Multicellular System with a Tunable DNA Patch on a Membrane Surface.
Nano Lett
; 24(1): 433-440, 2024 Jan 10.
Article
in En
| MEDLINE
| ID: mdl-38112415
ABSTRACT
Coordinating multiple artificial cellular compartments into a well-organized artificial multicellular system (AMS) is of great interest in bottom-up synthetic biology. However, developing a facile strategy for fabricating an AMS with a controlled arrangement remains a challenge. Herein, utilizing in situ DNA hybridization chain reaction on the membrane surface, we developed a DNA patch-based strategy to direct the interconnection of vesicles. By tuning the DNA patch that generates heterotrophic adhesion for the attachment of vesicles, we could produce an AMS with higher-order structures straightforwardly and effectively. Furthermore, a hybrid AMS comprising live cells and vesicles was fabricated, and we found the hybrid AMS with higher-order structures arouses efficient molecular transportation from vesicles to living cells. In brief, our work provides a versatile strategy for modulating the self-assembly of AMSs, which could expand our capability to engineer synthetic biological systems and benefit synthetic cell research in programmable manipulation of intercellular communications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Phenomena
/
Artificial Cells
Language:
En
Journal:
Nano Lett
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Estados Unidos