Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping.
Analyst
; 149(3): 707-711, 2024 Jan 29.
Article
in En
| MEDLINE
| ID: mdl-38230655
ABSTRACT
A PfAgo-G4 sensing platform exploiting G4 as a signal reporter was proposed, validated, and optimized. By introducing two mismatches at the Link strand, a universal nucleotide design rule was established for accurate single nucleotide polymorphism discrimination with PfAgo-G4. The FUT2 gene was then successfully and accurately genotyped using human buccal swab samples.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aptamers, Nucleotide
/
G-Quadruplexes
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Analyst
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Reino Unido