Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
ACS Infect Dis
; 10(2): 436-452, 2024 02 09.
Article
in En
| MEDLINE
| ID: mdl-38240689
ABSTRACT
Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein. Disruption of hfeA reduced H. influenzae manganese acquisition and was associated with decreased growth under aerobic conditions, impaired manganese-superoxide dismutase activity, reduced survival in macrophages, and changes in biofilm production in the presence of superoxide. Collectively, this work shows that HfeA contributes to H. influenzae manganese acquisition and virulence attributes. High conservation of the hfeABCD permease in Haemophilus species suggests that it may serve similar roles in other pathogenic Pasteurellaceae.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Transport Proteins
/
Haemophilus influenzae
Limits:
Humans
Language:
En
Journal:
ACS Infect Dis
/
ACS infect. dis
/
ACS infectious diseases
Year:
2024
Document type:
Article
Affiliation country:
Australia
Country of publication:
Estados Unidos