Cascaded elasto-inertial separation of malignant tumor cells from untreated malignant pleural and peritoneal effusions.
Lab Chip
; 24(4): 697-706, 2024 02 13.
Article
in En
| MEDLINE
| ID: mdl-38273802
ABSTRACT
Separation of malignant tumor cells (MTCs) from large background cells in untreated malignant pleural and peritoneal effusions (MPPEs) is critical for improving the sensitivity and efficiency of cytological diagnosis. Herein, we proposed a cascaded elasto-inertial cell separation (CEICS) device integrating an interfacial elasto-inertial microfluidic channel with a symmetric contraction expansion array (CEA) channel for pretreatment-free, high-recovery-ratio, and high-purity separation of MTCs from clinical MPPEs. First, the effects of flow-rate ratio, cell concentration, and cell size on separation performances in two single-stage channels were investigated. Then, the performances of the integrated CEICS device were characterized using blood cells spiked with three different tumor cells (MCF-7, MDA-MB-231, and A549 cells) at a high total throughput of 240 µL min-1. An average recovery ratio of â¼95% and an average purity of â¼61% for the three tumor cells were achieved. Finally, we successfully applied the CEICS device for the pretreatment-free separation of MTCs from clinical MPPEs of different cancers. Our CEICS device may provide a preparation tool for improving the sensitivity and efficiency of cytological examination.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microfluidic Analytical Techniques
Language:
En
Journal:
Lab Chip
Journal subject:
BIOTECNOLOGIA
/
QUIMICA
Year:
2024
Document type:
Article
Affiliation country:
China