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Prolonged near-infrared fluorescence imaging of microRNAs and proteases in vivo by aggregation-enhanced emission from DNA-AuNC nanomachines.
Wang, Ting; Jiang, Kai; Wang, Yifan; Xu, Limei; Liu, Yingqi; Zhang, Shiling; Xiong, Weiwei; Wang, Yemei; Zheng, Fenfen; Zhu, Jun-Jie.
Affiliation
  • Wang T; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Jiang K; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Wang Y; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Xu L; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Liu Y; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Zhang S; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Xiong W; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Wang Y; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Zheng F; School of Environmental & Chemical Engineering, Jiangsu University of Science and Technology Changhui Rd. 666 Zhenjiang Jiangsu 212003 China zhengfenfen@just.edu.cn.
  • Zhu JJ; State Key Laboratory of Analytical for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Xianlin Ave 163 Nanjing Jiangsu 210023 China jjzhu@nju.edu.cn.
Chem Sci ; 15(5): 1829-1839, 2024 Jan 31.
Article in En | MEDLINE | ID: mdl-38303939
ABSTRACT
Developing a comprehensive strategy for imaging various biomarkers (i.e., microRNAs and proteases) in vivo is an exceptionally formidable task. Herein, we have designed a deoxyribonucleic acid-gold nanocluster (DNA-AuNC) nanomachine for detecting tumor-related TK1 mRNA and cathepsin B in living cells and in vivo. The DNA-AuNC nanomachine is constructed using AuNCs and DNA modules that incorporate a three component DNA hybrid (TD) and a single-stranded fuel DNA (FD). Upon being internalized into tumor cells, the TK1 mRNA initiates the DNA-AuNC nanomachine through DNA strand displacement cascades, leading to the amplified self-assembly and the aggregation-enhanced emission of AuNCs for in situ imaging. Furthermore, with the aid of a protease nanomediator consisting of a mediator DNA/peptide complex and AuNCs (DpAuNCs), the DNA-AuNC nanomachine can be triggered by the protease-activated disassembly of the DNA/peptide complex on the nanomediator, resulting in the aggregation of AuNCs for in vivo protease amplified detection. It is worth noting that our study demonstrates the impressive tumor permeability and accumulation capabilities of the DNA-AuNC nanomachines via in situ amplified self-assembly, thereby facilitating prolonged imaging of TK1 mRNA and cathepsin B both in vitro and in vivo. This strategy presents a versatile and biomarker-specific paradigm for disease diagnosis.

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Chem Sci Year: 2024 Document type: Article Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Chem Sci Year: 2024 Document type: Article Country of publication: Reino Unido