Solution NMR Analysis of O-Glycopeptide-Antibody Interaction.
Methods Mol Biol
; 2763: 321-327, 2024.
Article
in En
| MEDLINE
| ID: mdl-38347421
ABSTRACT
O-Linked glycans potentially play a functional role in cellular recognition events. Recent structural analyses suggest that O-glycosylation can be a specific signal for a lectin receptor which recognizes both the O-glycan and the adjacent polypeptide region. Further, certain antibodies specifically bind to the O-glycosylated peptide. There is growing interest in the mechanism by which O-glycans on proteins are specifically recognized by lectins and antibodies. The recognition system may be common to many O-glycosylated proteins; however, there is limited 3D structural information on the dual recognition of glycan and protein. This chapter describes a solution NMR analysis of the interaction between MUC1 O-glycopeptide and anti-MUC1 antibody MY.1E12.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glycopeptides
/
Mucin-1
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Japón