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Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber.
Harris, Amy G; Schot, Vico; Carrabba, Michele; Iacobazzi, Dominga; Ghorbel, Mohamed T; Armstrong, James P K; Perriman, Adam W; Caputo, Massimo; Biglino, Giovanni; Bartoli-Leonard, Francesca.
Affiliation
  • Harris AG; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK. Electronic address: a.harris@bristol.ac.uk.
  • Schot V; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
  • Carrabba M; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
  • Iacobazzi D; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
  • Ghorbel MT; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
  • Armstrong JPK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK.
  • Perriman AW; School of Cellular and Molecular Medicine, Biomedical Sciences Building, University Walk, BS8 1TD Bristol, UK.
  • Caputo M; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiac Surgery, University Hospitals Bristol, NHS Foundation Trust, BS2 8HW Bristol, UK.
  • Biglino G; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiorespiratory Unit, Great Ormond Street Hospital for Children, NHS Foundation Trust, WC1N
  • Bartoli-Leonard F; Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK. Electronic address: f.bartoli-leonard@bristol.ac.uk.
STAR Protoc ; 5(1): 102899, 2024 Mar 15.
Article in En | MEDLINE | ID: mdl-38367231
ABSTRACT
Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold. For complete details on the use and execution of this protocol, please refer to Vafaee et al.1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Printing, Three-Dimensional / Heart Ventricles Limits: Animals / Child / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Printing, Three-Dimensional / Heart Ventricles Limits: Animals / Child / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article