Lymph node-biomimetic scaffold boosts CAR-T therapy against solid tumor.
Natl Sci Rev
; 11(4): nwae018, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38440217
ABSTRACT
The limited infiltration and persistence of chimeric antigen receptor (CAR)-T cells is primarily responsible for their treatment deficits in solid tumors. Here, we present a three-dimensional scaffold, inspired by the physiological process of T-cell proliferation in lymph nodes. This scaffold gathers the function of loading, delivery, activation and expansion for CAR-T cells to enhance their therapeutic effects on solid tumors. This porous device is made from poly(lactic-co-glycolic acid) by a microfluidic technique with the modification of T-cell stimulatory signals, including anti-CD3, anti-CD28 antibodies, as well as cytokines. This scaffold fosters a 50-fold CAR-T cell expansion in vitro and a 15-fold cell expansion in vivo. Particularly, it maintains long-lasting expansion of CAR-T cells for up to 30 days in a cervical tumor model and significantly inhibits the tumor growth. This biomimetic delivery strategy provides a versatile platform of cell delivery and activation for CAR-T cells in treating solid tumors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Natl Sci Rev
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
China