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Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1.
Erol, Ufuk; Sahin, Omer Faruk; Urhan, Osman Furkan; Atas, Ahmet Duran; Altay, Kursat.
Affiliation
  • Erol U; Department of Parasitology, Faculty of Veterinary Medicine, University of Sivas Cumhuriyet, Sivas 58140, Türkiye. Electronic address: ufukerol@cumhuriyet.edu.tr.
  • Sahin OF; Department of Parasitology, Faculty of Veterinary Medicine, University of Sivas Cumhuriyet, Sivas 58140, Türkiye.
  • Urhan OF; Republic of Türkiye Ministry of Agriculture and Forestry, General Directory Meat and Milk Board, Sivas Meat Processing Plant, Sivas 58380, Türkiye.
  • Atas AD; Department of Parasitology, Faculty of Medicine, University of Sivas Cumhuriyet, Sivas 58140, Türkiye.
  • Altay K; Department of Parasitology, Faculty of Veterinary Medicine, University of Sivas Cumhuriyet, Sivas 58140, Türkiye.
Comp Immunol Microbiol Infect Dis ; 107: 102154, 2024 Apr.
Article in En | MEDLINE | ID: mdl-38442543
ABSTRACT
Anaplasma phagocytophilum is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for A.phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum-like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. A.phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep Diseases / Cattle Diseases / Anaplasma phagocytophilum / Dog Diseases / Horse Diseases / Anaplasmosis Limits: Animals Country/Region as subject: Asia Language: En Journal: Comp Immunol Microbiol Infect Dis / Comp. immunol. microbiol. infect. dis / Comparative immunology, microbiology and infectious diseases Year: 2024 Document type: Article Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep Diseases / Cattle Diseases / Anaplasma phagocytophilum / Dog Diseases / Horse Diseases / Anaplasmosis Limits: Animals Country/Region as subject: Asia Language: En Journal: Comp Immunol Microbiol Infect Dis / Comp. immunol. microbiol. infect. dis / Comparative immunology, microbiology and infectious diseases Year: 2024 Document type: Article Country of publication: Reino Unido