Evaluation of human papillomavirus type 16 viral load and genome physical status in Iranian women with cervical disease.
Mol Biol Rep
; 51(1): 411, 2024 Mar 11.
Article
in En
| MEDLINE
| ID: mdl-38466465
ABSTRACT
BACKGROUND:
This study examined the viral load and physical status of the human papillomavirus 16 (HPV-16) genome in non-cancerous, precancerous and cancerous cervical lesions.METHODS:
Quantitative real-time PCR was performed to determine HPV-16 E2 and E6 viral load in 132 cervical specimens. E2/E6 viral load ratio was used to determine the physical status of HPV-16 genome.RESULTS:
E2 gene viral load was a significant (P < 0.001) predicting biomarker in differentiating non-cancerous from precancerous and cancerous samples. E6 gene viral load was significantly different between the groups (P < 0.001). The specificity and sensitivity of E2 and E6 in distinguishing SCC samples were 100% and 95% respectively.CONCLUSION:
HPV-16 viral load measured through E2 and E6 genes is a reliable indicator of lesion type.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Precancerous Conditions
/
Uterine Cervical Neoplasms
/
Oncogene Proteins, Viral
/
Papillomavirus Infections
Limits:
Female
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Mol Biol Rep
Year:
2024
Document type:
Article
Affiliation country:
Irán
Country of publication:
Países Bajos