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In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients.
Becker, Heike Ef; Kameli, Nader; Rustichelli, Alice; Heijnens, Britt Am; Stassen, Frank Rm; Penders, John; Jonkers, Daisy Mae.
Affiliation
  • Becker HE; Department of Gastroenterology/Hepatology, Division of Internal Medicine, NUTRIM School of Nutrition & Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
  • Kameli N; Department of Medical Microbiology, NUTRIM School of Nutrition & Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
  • Rustichelli A; Department of Medical Microbiology, NUTRIM School of Nutrition & Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
  • Heijnens BA; Department of Medical Microbiology, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia.
  • Penders J; Department of Gastroenterology/Hepatology, Division of Internal Medicine, NUTRIM School of Nutrition & Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
  • Jonkers DM; Department of Medical Microbiology, NUTRIM School of Nutrition & Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
Future Microbiol ; 19: 335-347, 2024 03.
Article in En | MEDLINE | ID: mdl-38470403
ABSTRACT

Aim:

This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function.

Methods:

FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing.

Results:

In vitro FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations.

Conclusion:

The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Crohn Disease Limits: Humans Language: En Journal: Future Microbiol / Future microbiol., (Print) / Future microbiology (Print) Journal subject: MICROBIOLOGIA Year: 2024 Document type: Article Affiliation country: Países Bajos Country of publication: Reino Unido
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Crohn Disease Limits: Humans Language: En Journal: Future Microbiol / Future microbiol., (Print) / Future microbiology (Print) Journal subject: MICROBIOLOGIA Year: 2024 Document type: Article Affiliation country: Países Bajos Country of publication: Reino Unido