Viral reprogramming of host transcription initiation.
Nucleic Acids Res
; 52(9): 5016-5032, 2024 May 22.
Article
in En
| MEDLINE
| ID: mdl-38471819
ABSTRACT
Viruses are master remodelers of the host cell environment in support of infection and virus production. For example, viruses typically regulate cell gene expression through modulating canonical cell promoter activity. Here, we show that Epstein Barr virus (EBV) replication causes 'de novo' transcription initiation at 29674 new transcription start sites throughout the cell genome. De novo transcription initiation is facilitated in part by the unique properties of the viral pre-initiation complex (vPIC) that binds a TATT[T/A]AA, TATA box-like sequence and activates transcription with minimal support by additional transcription factors. Other de novo promoters are driven by the viral transcription factors, Zta and Rta and are influenced by directional proximity to existing canonical cell promoters, a configuration that fosters transcription through existing promoters and transcriptional interference. These studies reveal a new way that viruses interact with the host transcriptome to inhibit host gene expression and they shed light on primal features driving eukaryotic promoter function.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Replication
/
Herpesvirus 4, Human
/
Epstein-Barr Virus Infections
/
Transcription Initiation, Genetic
Limits:
Humans
Language:
En
Journal:
Nucleic Acids Res
/
Nucleic acids res
/
Nucleic acids research
Year:
2024
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Reino Unido