Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells.
Dokl Biol Sci
; 513(Suppl 1): S41-S44, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-38472685
ABSTRACT
The effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in developing countries according to the World Health Organization. Various technologies have recently been proposed to restore the damaged surface of the cornea. Use of biodegradable silk-based materials, including recombinant analogs of the spider silk protein spidroin, is an important avenue of research in the field of wound healing and corneal regeneration. Spidroins are well known for their optimal balance of strength and elasticity. Given their biological compatibility, lack of immunogenicity, and biodegradability, spidroins provide a biomaterial for tissue engineering and regenerative medicine. HGs based on RS rS2/12-RGDS were therefore tested for cytotoxicity toward isolated corneal epithelial cells and keratocytes with regard to possible changes in cell phenotype and migratory activity. A promising outlook and therapeutic potential were demonstrated for RS-based HGs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibroins
Limits:
Humans
Language:
En
Journal:
Dokl Biol Sci
Year:
2023
Document type:
Article
Affiliation country:
Rusia
Country of publication:
Estados Unidos