HIV-Tocky system to visualize proviral expression dynamics.
Commun Biol
; 7(1): 344, 2024 Mar 20.
Article
in En
| MEDLINE
| ID: mdl-38509308
ABSTRACT
Determinants of HIV-1 latency establishment are yet to be elucidated. HIV reservoir comprises a rare fraction of infected cells that can survive host and virus-mediated killing. In vitro reporter models so far offered a feasible means to inspect this population, but with limited capabilities to dissect provirus silencing dynamics. Here, we describe a new HIV reporter model, HIV-Timer of cell kinetics and activity (HIV-Tocky) with dual fluorescence spontaneous shifting to reveal provirus silencing and reactivation dynamics. This unique feature allows, for the first time, identifying two latent populations a directly latent, and a recently silenced subset, with the latter having integration features suggestive of stable latency. Our proposed model can help address the heterogeneous nature of HIV reservoirs and offers new possibilities for evaluating eradication strategies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
HIV Infections
/
Proviruses
Limits:
Humans
Language:
En
Journal:
Commun Biol
/
Commun. biolog
/
Communications biology
Year:
2024
Document type:
Article
Affiliation country:
Japón
Country of publication:
Reino Unido