Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform.
Biosens Bioelectron
; 254: 116218, 2024 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-38518559
ABSTRACT
Biodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated nanowire platform and applied it with a sandwich fluorophore-linked immunosorbent well plate assay (FLISA) to detect Dengue NS1 in urine. For the platform, we fabricated zinc oxide (ZnO) nanowires to provide a high surface area and then coated them with gold nanoparticles (ZnO/Au nanowires) to simply modify the Dengue NS1 antibody and enhance the fluorescence intensity. Our platform employs a sandwich FLISA that exhibits high sensitivity, specifically detecting Dengue NS1 with a limit of detection (LOD) of 1.35 pg/mL. This LOD was 4500-fold lower than the LOD of a commercially available kit for Dengue NS1 enzyme-linked immunosorbent assay. We believe that our ZnO/Au nanowire platform has the potential to revolutionize the field of non-invasive diagnostics for dengue.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zinc Oxide
/
Biosensing Techniques
/
Dengue
/
Dengue Virus
/
Metal Nanoparticles
/
Nanowires
Limits:
Humans
Language:
En
Journal:
Biosens Bioelectron
/
Biosens. bioelectron
/
Biosensors and bioelectronics
Journal subject:
BIOTECNOLOGIA
Year:
2024
Document type:
Article
Country of publication:
Reino Unido