Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures.
Methods Enzymol
; 695: 221-232, 2024.
Article
in En
| MEDLINE
| ID: mdl-38521586
ABSTRACT
Cytosine rich sequences can form intercalated, i-motif DNA structures stabilized by hemi-protonated cytosinecytosine base pairing. These sequences are often located in regulatory regions of genes such as promoters. Ligands targeting i-motif structures may provide potential leads for treatments for genetic disease. The focus on ligands interacting with i-motif DNA has been increasing in recent years. Here, we describe the fluorescent intercalator displacement (FID) assay using thiazole orange binding i-motif DNA and assess the binding affinity of a ligand to the i-motif DNA by displacing thiazole orange. This provides a time and cost-effective high throughput screening of ligands against secondary DNA structures for hit identification.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Intercalating Agents
Language:
En
Journal:
Methods Enzymol
Year:
2024
Document type:
Article