Cryo-EM sample preparation for high-resolution structure studies.
Acta Crystallogr F Struct Biol Commun
; 80(Pt 4): 74-81, 2024 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-38530656
ABSTRACT
High-resolution structures of biomolecules can be obtained using single-particle cryo-electron microscopy (SPA cryo-EM), and the rapidly growing number of structures solved by this method is encouraging more researchers to utilize this technique. As with other structural biology methods, sample preparation for an SPA cryo-EM data collection requires some expertise and an understanding of the strengths and limitations of the technique in order to make sensible decisions in the sample-preparation process. In this article, common strategies and pitfalls are described and practical advice is given to increase the chances of success when starting an SPA cryo-EM project.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Cryoelectron Microscopy
Language:
En
Journal:
Acta Crystallogr F Struct Biol Commun
/
Acta cryst. F, Struct. biolog. commun
/
Acta crystallographica. Section F, Structural biology communications
Year:
2024
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Estados Unidos